Development and Characterization of Bioinspired Cationic Lipid Nanocarriers for Enhanced Anti-Cancer Vaccine Delivery and Tumor Inhibition: In Vitro and In Vivo Evaluation

Abstract

This study develops and characterizes bioinspired nanocarriers for anti-cancer vaccination delivery and efficacy. Cationic lipid nanoparticles (CLP) were combined with cancer cell membrane fractions to generate a new Cancer Membrane Delivery System. DLS and TEM were used to analyse the nanoparticles' size distribution, zeta potential, and morphology. To ensure safety and efficacy, biocompatibility, cytotoxicity, and nucleic acid encapsulation efficiency were tested. In vitro and in vivo studies showed nanoparticles' high cellular absorption and tumor growth inhibition. Immunological and blood parameter analyses suggested immunomodulation. Statistical study using ANOVA showed substantial immunological and blood parameter differences between nanoparticle formulations. This work shows that bioinspired nanocarriers may be promising cancer treatment platforms.